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Optical Microscopy


The microraman spectrometer and the bioscope Catalyst

The microraman spectrometer combined with the bioscope allow simultaneous measurements of molecular dynamics (including surface mapping)  and surface physical properties using a multimode atomic force microscope (incorporating latest PeakForce Tapping innovation).

Important parameters:

Temperature range -195 0C till  +600 0C
Laser wave length:785 nm,633 nm, 514 nm

  • Spectral resolution better than 1 cm-1
  • Spectrometer mode: surface mapping (streamline imaging),
  • Depth mapping
Bioscope scan range XY>150µm Z>20 µm Modes:
  • AFM (multimode system)
  • Life cell imaging

 

Application: biology, material science.


Raman spectra collected from the multiwall carbon nanotube shows three characteristic bands: G, D and RBM. Band G is related to bulk carbon structure, band D to disorder in the carbon nanotube and band RBM to a radial breathing mode. A wave number of the RBM depends on diameter of the tube.
 


A microraman spectrometer and a NT-MDT SNOM microscope

The microraman spectrometer combined with the NT_MDT SNOM allow simultaneous measurements of molecular dynamics (including surface mapping) and physical properties which can be investigated using light e.g. surface plasmons, optical transmission and reflection. Share forces, multimode atomic force microscope as well as thermal properties measurements are also available in this set up. 

Important parameters:

  • Laser wave length:633 nm,514 nm, 488 nm
  • Spectral resolution better than 1 cm-1
  • Spectrometer mode: surface mapping (point imaging),
  • Depth mapping
SNOM scan range XY=100x100 µm Z=10 µm
Modes:
  • Share force
  • Transmission
  • Reflection
  • AFM (multimode system)
  • Thermal conductivity

Application: material science, biology


Presented in Fig.2 result of the surface mapping mode shows a nonuniform graphene sample.
The 2D peak related to number of layers of a graphene flake, mapped over a sample surface, reflects change in the sample’s thickness.

 


Optical microscopy

Microscopes and equipment

Zeiss:

  • scanning fluorescence microscope LSM 780 NLO,
  • halogen + fluorescence lamp + filters
  • CW lasers: 405, 458, 488, 514, 561, 633 nm
  • Two photon excitation (Chameleon 680-1080nm, 140 fs),
  • spectral module,
  • fluorescence correlation spectroscopy (FCS – ConfoCor 3)

Olympus:

  • scanning fluorescence microscope FV 1000,
  • halogen + fluorescence lamp + filters
  • CW lasers: 405, 457, 473, 488, 514, 561, 638 nm
  • spectral module,
  • FLIM (485, 635 nm),
  • TIRF (Andor camera),
  • FCS (Picoquant)

Leica:

  • scanning fluorescence microscope
  • halogen + fluorescence lamp + filters
  • STED (super-resolution),
  • white laser 470 – 670 nm,
  • CW lasers: Ar, 458, 476, 488, 496, 514 nm
  • spectral module,
  • incubation chamber (temperature, CO2)
  • FCS (Picoquant)

The optical microscopy lab provides instruments for studying the structure, dynamics and optical properties of matter in the nano- and micro-scale.

  • Each of the three microscopes may serve as a regular optical microscope operating with magnification of ~1000x both in the bright field and fluorescence mode.
  • Each of the three microscopes may work in the mode of the laser scanning fluorescence microscope in order to obtain precise images of fluorescently labelled samples. A broad spectrum of excitation wavelenghts is available.
  • Each of the three microscopes may perform spectral measurements which allows measurements of fluorescence spectrum of single molecules or of sub-micron regions of the sample.
  • Each of the three microscopes can measure the kinetics of fluorescence fluctuations (fluorescence correlation spectroscopy – FCS) which allows for measurements of diffusion coefficients of fluorescently labelled particles in extremely low concentrations (nanomolar).
  • Olympus microscope has two special options: FLIM (fluorescence life-time imaging) and TIRF (total internal reflection imaging).
  • Zeiss microscope is equipped with a tunable femtosecond infrared laser used for two-photon excitation.
  • Leica microscope is equipped with a tunable visible laser (“white laser”) and the STED super-resolution option (stimulated emission depletion) extending the resolution to ~40 nm range.
 

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