Optical Microscopy
Catalyst Raman Spectrometer and Scanning Microscope
By coupling a Raman spectrometer (microraman) to a scanning probe microscope (SPM), it is possible to simultaneously measure molecular dynamics by Raman light scattering (including linear mapping of excitations on the sample surface) and to study the physical properties of surfaces using measurement modalities available on modern atomic force microscopes (AFMs), including peak force tapping.
Key parameters of the measurement system
- Raman spectrometer (micro-Raman)
- Temperature range in which measurements can be made: -195 0C to +600 0CD
- Laser wavelengths: 785 nm, 633 nm, 514 nm
- Resolution less than 1 cm-1
- Measuring modalities: excitation mapping on the sample surface (streamline imaging), excitation mapping at a specific laser beam penetration depth
- Catalyst scanning microscope
- Maximum scanning area size XY>150µm
- Scanning object height Z>20 µm
- Measuring modalities: multimode AFM, live cell imaging
Application area
- Biology
- Nanotechnology
- Materials Science
NT_MDT SNOM Raman Spectrometer and Scanning Microscope
By coupling a Raman spectrometer (microraman) to the NT_MDT SNOM microscope, molecular dynamics can be studied simultaneously using Raman light scattering (including spot mapping of excitations on the sample surface) and physical properties such as light transmission and reflection, mechanical properties and thermal properties using a multi-mode atomic force microscope.
Key parameters of the measurement system
- Raman spectrometer (micro-Raman)
- Temperature range in which measurements can be made: -195 deg C to +600 deg C
- Laser wavelengths: 488 nm, 633 nm, 514 nm
- Resolution less than 1 cm-1
- Measuring methods: excitation mapping on the sample surface (point imaging), excitation mapping at a specific penetration depth of the laser beam
NT-MDT SNOM Microscope
- Maximum size of scanned area XY 100x100µm
- Height of scanned objects Z=10 µm
- Measuring methods: shear forces, reflection and optical transmission, multimode AFM, temperature distribution measurement.
Application area
- Biology
- Nanotechnology
- Materials Science
Microscopes and equipment
Zeiss
- Scanning Fluorescence Microscope LSM 780 NLO
- Halogen + Fluorescence Lamp + Filters
- CW lasers 405, 458, 488, 514, 561, 633 nm
- Biphoton excitation (Chameleon 680-1080nm, 140 fs)
- Spectral attachment
- Fluorescence correlation spectroscopy (FCS - ConfoCor 3)
Olympus
- Fluorescence Scanning Microscope FV 1000
- Halogen + Fluorescence Lamp + Filters
- CW Lasers 405, 457, 473, 488, 514, 561, 638 nm
- Spectral imager
- FLIM (485, 635 nm)
- TIRF (Andor camera)
- FCS (Picoquant)
Leica
- Scanning Fluorescence Microscope
- Halogen + fluorescence lamp + filters
- STED (super resolution)
- White laser 470 - 670 nm,CW laser: Ar, 458, 476, 488, 496, 514 nm
- Spectral imager
- Incubation system for survival studies (temperature, CO2)
- FCS (Picoquant)
The Optical Microscopy Laboratory brings together instruments to study the structure, dynamics and optical properties of matter at the nano- and micrometer scale
- Each of the three microscopes can be used to view slides at up to ~1000x magnification in transmitted light or fluorescence (lamp) mode.
- Each of the three microscopes can operate in laser scanning confocal mode to produce precise images of fluorescently labelled samples. Almost the entire visible spectrum of the excitation band is available.
- Each of the three microscopes has a spectral attachment to measure the fluorescence spectrum of individual molecules or sub-micron areas.
- Each of the three microscopes can measure fluorescence fluctuation kinetics (fluorescence correlation spectroscopy - FCS), which allows the determination of diffusion coefficients of fluorescently labelled molecules at very low concentrations, e.g. to study the binding constant of a ligand to a receptor.