Important parameters:

• Laser wave length:633 nm,514 nm, 488 nm
• Spectral resolution better than 1 cm-1
• Spectrometer mode: surface mapping (point imaging),
• Depth mapping

• Share force
• Transmission
• Reflection
• AFM (multimode system)
• Thermal conductivity

Application: material science, biology

Presented in Fig.2 result of the surface mapping mode shows a nonuniform graphene sample.
The 2D peak related to number of layers of a graphene flake, mapped over a sample surface, reflects change in the sample’s thickness.
 

The microraman spectrometer combined with the bioscope allow simultaneous measurements of molecular dynamics (including surface mapping)  and surface physical properties using a multimode atomic force microscope (incorporating latest PeakForce Tapping innovation).

Important parameters:

Temperature range -195 0C till  +600 0C
Laser wave length:785 nm,633 nm, 514 nm

• Spectral resolution better than 1 cm-1
• Spectrometer mode: surface mapping (streamline imaging),
• Depth mapping

• AFM (multimode system)
• Life cell imaging

Application: biology, material science.

Raman spectra collected from the multiwall carbon nanotube shows three characteristic bands: G, D and RBM. Band G is related to bulk carbon structure, band D to disorder in the carbon nanotube and band RBM to a radial breathing mode. A wave number of the RBM depends on diameter of the tube.
 

Microscopes and equipment

Zeiss:

• scanning fluorescence microscope LSM 780 NLO,
• halogen + fluorescence lamp + filters

• CW lasers: 405, 458, 488, 514, 561, 633 nm
• Two photon excitation (Chameleon 680-1080nm, 140 fs),
• spectral module,
• fluorescence correlation spectroscopy (FCS – ConfoCor 3)

Olympus:

• scanning fluorescence microscope FV 1000,

• halogen + fluorescence lamp + filters

• CW lasers: 405, 457, 473, 488, 514, 561, 638 nm
• spectral module,

• FLIM (485, 635 nm),
• TIRF (Andor camera),
• FCS (Picoquant)

Leica:

• scanning fluorescence microscope

• halogen + fluorescence lamp + filters

• STED (super-resolution),
• white laser 470 – 670 nm,
• CW lasers: Ar, 458, 476, 488, 496, 514 nm
• spectral module,
• incubation chamber (temperature, CO2)
• FCS (Picoquant)

The optical microscopy lab provides instruments for studying the structure, dynamics and optical properties of matter in the nano- and micro-scale.

• Each of the three microscopes may serve as a regular optical microscope operating with magnification of ~1000x both in the bright field and fluorescence mode.
• Each of the three microscopes may work in the mode of the laser scanning fluorescence microscope in order to obtain precise images of fluorescently labelled samples. A broad spectrum of excitation wavelenghts is available.
• Each of the three microscopes may perform spectral measurements which allows measurements of fluorescence spectrum of single molecules or of sub-micron regions of the sample.
• Each of the three microscopes can measure the kinetics of fluorescence fluctuations (fluorescence correlation spectroscopy – FCS) which allows for measurements of diffusion coefficients of fluorescently labelled particles in extremely low concentrations (nanomolar).
• Olympus microscope has two special options: FLIM (fluorescence life-time imaging) and TIRF (total internal reflection imaging).
• Zeiss microscope is equipped with a tunable femtosecond infrared laser used for two-photon excitation.
• Leica microscope is equipped with a tunable visible laser (“white laser”) and the STED super-resolution option (stimulated emission depletion) extending the resolution to ~40 nm range.